Coding

Part:BBa_K771106

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-24)

GBDligand

components of interacting proteins

Usage and Biology

12SJTU GBDdl.gif

The GBD ligand along with its domain(Part:BBa_K771105) comes from Rattus rattus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.

Related Parts

Part:BBa_K771105, GBD domain


Part:BBa_K771004, Membrane Anchor 4, containing GBD domain


Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGBDligand